Extended Data Fig. 6: Assessment of off-target selectivity for the best V-SYNTHES CB2 hits.
From: Synthon-based ligand discovery in virtual libraries of over 11 billion compounds
(a-c) Screening of compounds 673, 610 and 523 at 10 µM concentrations in GPCRome-Tango assays for >300 receptors. Dopamine D2 (DRD2) and 100 nM Quinpirole served as an assay control. The data are presented as mean ± SEM (n = 4) and the values of fold of basal > 3 are marked as significant hits. (d-o) Follow-up dose-response curves for targets with >3 fold increased activity. Known agonists or antagonist that showed activity served as positive controls. The data points are presented as mean ± SEM with n = 3 independent experiments, each assay carried out in triplicate.
